myelin associated glycoprotein  (Bioss)

Journal: Experimental and therapeutic medicine

Article Title: MicroRNA-210 contributes to peripheral nerve regeneration through promoting the proliferation and migration of Schwann cells.

doi: 10.3892/etm.2017.4869

Figure Lengend Snippet: Figure 4. miR‑210 has an effect on the protein levels of GAP‑43, MAG and MBP. (A and B) After transfection with miR‑210 mimics or miR‑210 inhibitor, the protein levels of GAP‑43 were detected by western blot analysis. Relative protein levels were calculated using β‑actin as an internal reference. (C and D) The protein levels of MBP were detected by western blot after transfection with miR‑210 mimics or miR‑210 inhibitor. β‑actin was used as an internal reference. (E and F) After transfection with miR‑210 mimics or miR‑210 inhibitor, the protein levels of MAG were detected by western blot using β‑actin as an internal reference. All experiments were repeated three times and values are expressed as the mean ± standard deviation. **P<0.01, ***P<0.001. miR, microRNA; MAG, myelin‑associated glycoprotein; MBP, myelin basic protein; GAP‑43, growth‑associated protein 43; NC, negative control.

Article Snippet: The membranes were blocked with 5% skimmed milk, followed by incubation with primary antibodies against growth-associated protein-43 (GAP-43; 1:200 dilution; Santa Cruz Biotechnologies, Inc., Dallas, TX, USA; cat. no. sc-33705), myelin-associated glycoprotein (MAG; 1:500 dilution; Bioss, Beijing, China; cat no. bs-0257R), myelin basic protein (MBP; 1:500 dilution; Bioss; cat. no. bs-0380R) and β‐actin (1:1,000, Santa Cruz Biotechnologies, Inc.) at 4 ̊C overnight.

Techniques: Transfection, Western Blot, Standard Deviation, Negative Control

Journal: Cell reports

Article Title: PARP1-mediated PARylation activity is essential for oligodendroglial differentiation and CNS myelination

doi: 10.1016/j.celrep.2021.109695

Figure Lengend Snippet:

Article Snippet: Rabbit polyclonal MAG , Millipore , Cat#AB1567; RRID: AB_2110397.

Techniques: Binding Assay, Purification, Control, Recombinant, Electron Microscopy, Protein Extraction, Lysis, Protease Inhibitor, Transfection, Staining, Bicinchoninic Acid Protein Assay, Magnetic Beads, SYBR Green Assay, Western Blot, Knock-Out, Software

Journal: CNS Neuroscience & Therapeutics

Article Title: Proteomic analysis of the effects of caffeine in a neonatal rat model of hypoxic‐ischemic white matter damage

doi: 10.1111/cns.13834

Figure Lengend Snippet: Top 10 upregulated and downregulated proteins in Sham group and Caffeine group compared with the HI group

Article Snippet: The membranes were blocked with 5% fat‐free milk and then incubated with the following primary antibodies: rabbit anti‐MBP monoclonal antibodies (1:1000), mouse anti‐SITR2 monoclonal antibodies (1:2500), rabbit anti‐myelin‐associated glycoprotein (MAG) polyclonal antibodies (1:1000; cat. no. 14386‐1‐AP; Proteintech), rabbit anti‐myelin proteolipid protein (PLP) polyclonal antibodies (1:1000; cat. no. DF13282; Affinity Biosciences), rabbit anti‐PSD‐95 polyclonal antibodies (1:1000), and rabbit anti‐synaptophysin polyclonal antibodies (1:5000); rabbit anti‐β‐tubulin polyclonal antibodies (1:5000; cat. no. 10068‐1‐AP; Proteintech) was used as the loading control.

Techniques: Sequencing, Histone Deacetylase Assay, RNA Binding Assay